山羊STAT3基因克隆、生物信息学分析及甲基化修饰研究

doi:10.11843/j.issn.0366-6964.2016.03.006

Abstract

Abstract:

The aim of this study was to clone and analyze the entire coding sequence of goat STAT3 gene as well as to detect the STAT3 gene methylation modification and analyze its association with goat body weight using reverse transcription-polymerase chain reaction(RT-PCR)，TA cloning，bioinformatics and bisulfite sequencing(BSP) methods.The results showed that the entire coding sequence of goat STAT3 gene was 2 313 bp in length，and encoded 770 amino acids.The shared amino acid sequence consistency between goat(capra hircus) and sheep(Ovis aries)，bovine(Bos Taurus)，boar(Sus scrofa)，mouse(Mus musculus），rat(Rattus norvegicus)，human(Homo sapiens) were 99.7%，99.6%，99.4%，99.2%，99.4%，99.5%，respectively.The analysis of the methylation difference in the promoter region of goat STAT3 gene between high body weight group and low body weight group demonstrated that the goats with high body weight had higher methylation level than those goats with low body weight(P=0.020).This finding indicated that the methylation modification of STAT3 gene had significant effect on body weight in goat，which could become the epigenetic marker in marker-assisted selection(MAS).These results would provide scientific data for studying genetic and epigenetic mechanism for meat production in goat.

CLC Number:

S827
S813.3

JIA Wen-chao，LIU Liang-liang，WU Xian-feng，ZHAO Zhao-yan，WANG Ke，WANG Mao，GAO Jing，WANG Li-qiang，CHEN Hong，PAN Chuan-ying，LAN Xian-yong. Molecular Cloning，Bioinformatics Analysis and Methylation Modification of Goat STAT3 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 457-466.

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Molecular Cloning，Bioinformatics Analysis and Methylation Modification of Goat STAT3 Gene

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